The RWD domain of Yih1 was formerly modeled on the mouse Gcn2 RWD structure and unveiled conserved residues in helix H2 and H3 that are probably uncovered to the solvent and consequently might act as docking web-sites for Yih1 binding partners
From all Yih1 fragments, GST-Yih1, GST-Yih1, and GST-Yih1 sure Cdc28 the strongest (Fig 9E), suggesting that amino acids 6832 contain the big Cdc28 binding determinant. Curiously, these Yih1 fragments bind…