Product: AM32832PU-TBcl-2 (41-54) antibody
Quantity: 20 µg
Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 1428-67-7 Product: DPN
Shipping to: Worldwide
Immunogen: Synthetic peptide corresponding to amino acids 41-54 of Human Bcl-2AA Sequence:GAAPAPGIFSSQPG-CysGeneID:596Remarks:This sequence is 100% conserved in the Human alpha (239 aa) and beta (205 aa) isoforms.
Application: ELISA: For coating, Use Antibody without BSA.Western blot: 0.5-1 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunofluorescence: 1-2 µg/ml. Flow Cytometry: 0.5-1µg/106 cells.Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µ
Background: Bcl-2 (Bcl2) is the founding member of the Bcl-2 family. Family members have pivotal roles in regulating apoptosis or death signaling pathways through their control of mitochondrial permeability and cytochrome release (reviewed in Anvekar, 2011; Martinou
Concentration: 1.0 mg/ml
Storage: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 10mM PBS
Preservatives: 0.05% Sodium Azide
State: Liquid purified IgG fraction from Bioreactor ConcentratePurified
Specifictiy: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2-apha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. The Monoclonal 8C8 antibody has been extensively used to monitor the status of Bcl-2 expression in a myriad of