Purity: > 95 % as determined by SDS-PAGE. Endotoxin: Biological Activity: Measured by its ability to inhibit the IL-6R enhancement of IL-6 activity on M1 mouse myeloid leukemia cell. The ED50for this effect is typically 0.2-0.8 g/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6. Protein construction: A DNA sequence encoding the extracellular domain (Met 1-Ile 618) of human IL6ST (NP_002175.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. NP No. NP_002175.2 Expressing host: HEK293 Cells Species: Human Glu 23 Molecular Weight: The recombinant human IL6ST/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 844 amino acids and has a predicted molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL6ST/Fc monomer is approximately 125-140 kDa due to glycosylation. Buffer solution: Lyophilized from sterile PBS, pH 7.4
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Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. Transportation method: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. stability & Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80. Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Redissolution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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