Species: Human Expressing host: HEK293 cells Description: Recombinant Human PDGFR-beta/CD140b(E241D) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu33-Phe530 (Glu241Asp)) of human PDGFRB/CD140b (Accession #NP_002600.1) fused with an Fc, 6His tag at the C-terminus. Tag: C-hFc&His Purity: > 90% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PDGF-B at 2 g/mL (100 L/well) can bind recombinant Human PDGFRB. The EC50of Human PDGFRB is 30.48 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The protein is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. GeneID 5159 Swiss Prot P09619 Alias: CD140B;IBGC4;IMF1;JTK12;KOGS;PDGFR;PDGFR-1;PDGFR1;PENTT;PDGF Receptor beta;PDGFRB;PDGFR betaMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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