Species: Human Expressing host: HEK293 cells Description: Recombinant Human CSF1R/M-CSF R/CD115 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Glu512) of human M-CSF R/CSF1R/CD115 (Accession #NP_005202.2) fused with a Fc, 6His tag at the C-terminus. Tag: C-hFc&His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CSF1 at 1g/mL (100 L/well) can bind Human CSF1R with a linear range of 0.01-7.8 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for 12 months.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The recombinant human CSF1R/GST chimera consists of 667 amino acids with a molecular weight of 76 KDa. It migrates as an about 75 KDa band in SDS-PAGE under reducing conditions.M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages.This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer”s disease and after brain injuries. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression. GeneID 1436 Swiss Prot P07333 Alias: CSF1R;C-FMS;CD115;CSF-1R;CSFR;FIM2;FMS;HDLS;M-CSF-R;BANDDOSMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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